医療メモ
医療に関する自分のためのメモです
専門的なので、おすすめできません。
2008/6/9
「新種のCB中幹細胞かSPか」
OCT4とかSSEA-１は胎児組織なら大体出ている。
この集団がside population （SP）との異同が問題となろう
SPもたしかCD45-の集団があったはずだ。（参考資料では半分４５ー）
結局、ESとまでは行かないが、骨髄、CBとも上位の幹細胞があるらしいことがだんだんはっきりしてきた。verfailleは嘘ついていなかったわけだ。
彼女はMSCと言っているが、形態は細胞サイクルや場所でで変わりうる。
おそらく各臓器も同様の細胞がいるのでしょう。
先述のCB移植によるコラーゲン遺伝疾患の治療はこの辺を踏まえているわけだ。
素粒子が周期律表がかけるくらい増えてきているように、幹細胞もどんどん増えそうな勢い。ありゃま！
Leukemia. 2007 Feb;21(2):297-303. Epub 2006 Nov 30.Click here to read
Morphological and molecular characterization of novel population of CXCR4+ SSEA-4+ Oct-4+ very small embryonic-like cells purified from human cord blood: preliminary report.
Recently, we purified from adult murine bone marrow (BM) a population of CXCR4(+), Oct-4(+) SSEA-1(+), Sca-1(+) lin(-) CD45(-) very small embryonic-like (VSEL) stem cells and hypothesized that similar cells could be also present in human cord blood (CB). Here, we report that by employing a novel two-step isolation procedure -- removal of erythrocytes by hypotonic lysis combined with multiparameter sorting -- we could isolate from CB a population of human cells that are similar to murine BM-derived VSELs, described previously by us. These CB-isolated VSELs (CB-VSEL) are very small (3-5 micro m) and highly enriched in a population of CXCR4(+)AC133(+)CD34(+)lin(-) CD45(-) CB mononuclear cells, possess large nuclei containing unorganized euchromatin and express nuclear embryonic transcription factors Oct-4 and Nanog and surface embryonic antigen SSEA-4. Further studies are needed to see if human CB-isolated VSELs similar to their murine BM-derived counterparts are endowed with pluripotent stem cell properties.
参考：BM-SPの表面抗原
Bone Marrow-derived Side Population Cells are Capable of Functional Cardiomyogenic Differentiation.
Yoon J, Choi SC, Park CY, Choi JH, Kim YI, Shim WJ, Lim DS.
Department of Cardiology, College of Medicine, Korea University, Seoul 136-705, Korea.
It has been reported that bone marrow (BM)-side population (SP) cells, with hematopoietic stem cell activity, can transdifferentiate into cardiomyocytes and contribute to myocardial repair. However, this has been questioned by recent studies showing that hematopoietic stem cells (HSCs) adopt a hematopoietic cell lineage in the ischemic myocardium. The present study was designed to investigate whether BM-SP cells can in fact transdifferentiate into functional cardiomyocytes. Phenotypically, BM-SP cells were 19.59% (+/-)9.00 CD14+, 8.22% (+/-)2.72 CD34+, 92.93% (+/-)2.68 CD44+, 91.86% (+/-)4.07 CD45+, 28.48% (+/-)2.24 c-kit+, 71.09% (+/-)3.67 Sca-1+. Expression of endothelial cell markers (CD31, Flk-1, Tie-2 and VEGF-A) was higher in BM-SP cells than whole BM cells. After five days of co-culture with neonatal cardiomyocytes, 7.2% (+/-)1.2 of the BM-SP cells expressed sarcomeric -actinin as measured by flow cytometry. Moreover, BM-SP cells co-cultured on neonatal cardiomyocytes fixed to inhibit cell fusion also expressed sarcomeric -actinin. The co-cultured BM-SP cells showed neonatal cardiomyocyte-like action potentials of relatively long duration and shallow resting membrane potential. They also generated calcium transients with amplitude and duration similar to those of neonatal cardiomyocytes. These results show that BM-SP cells are capable of functional cardiomyogenic differentiation when co-cultured with neonatal cardiomyocytes.
＜引用終わり＞
それで、なんで素粒子に関心があるかと言うと、量子力学の観測問題というのがあって、観測すると位置が決まる、観測前はどこにおるか決められん（わからん、でなく、きめられんです）
これは幹細胞に似てるわけ。ヒエラルキー論では決定論的なんですが、実体としての幹細胞は場の関数でもあり、観測の関数でもあり、時間の関数でもあり、決定論にさからっとるわけだ。「何になろうと俺の勝手や、もんく言うな」、と言ってるみたい。
素朴な物心２言論が量子概念ですっ飛んだように、まさに幹細胞概念も秦の始皇帝に始まるヒエラルキードグマに反抗している！？
だから医学も、新時代なのだわ。
この集団がside population （SP）との異同が問題となろう
SPもたしかCD45-の集団があったはずだ。（参考資料では半分４５ー）
結局、ESとまでは行かないが、骨髄、CBとも上位の幹細胞があるらしいことがだんだんはっきりしてきた。verfailleは嘘ついていなかったわけだ。
彼女はMSCと言っているが、形態は細胞サイクルや場所でで変わりうる。
おそらく各臓器も同様の細胞がいるのでしょう。
先述のCB移植によるコラーゲン遺伝疾患の治療はこの辺を踏まえているわけだ。
素粒子が周期律表がかけるくらい増えてきているように、幹細胞もどんどん増えそうな勢い。ありゃま！
Leukemia. 2007 Feb;21(2):297-303. Epub 2006 Nov 30.Click here to read
Morphological and molecular characterization of novel population of CXCR4+ SSEA-4+ Oct-4+ very small embryonic-like cells purified from human cord blood: preliminary report.
Recently, we purified from adult murine bone marrow (BM) a population of CXCR4(+), Oct-4(+) SSEA-1(+), Sca-1(+) lin(-) CD45(-) very small embryonic-like (VSEL) stem cells and hypothesized that similar cells could be also present in human cord blood (CB). Here, we report that by employing a novel two-step isolation procedure -- removal of erythrocytes by hypotonic lysis combined with multiparameter sorting -- we could isolate from CB a population of human cells that are similar to murine BM-derived VSELs, described previously by us. These CB-isolated VSELs (CB-VSEL) are very small (3-5 micro m) and highly enriched in a population of CXCR4(+)AC133(+)CD34(+)lin(-) CD45(-) CB mononuclear cells, possess large nuclei containing unorganized euchromatin and express nuclear embryonic transcription factors Oct-4 and Nanog and surface embryonic antigen SSEA-4. Further studies are needed to see if human CB-isolated VSELs similar to their murine BM-derived counterparts are endowed with pluripotent stem cell properties.
参考：BM-SPの表面抗原
Bone Marrow-derived Side Population Cells are Capable of Functional Cardiomyogenic Differentiation.
Yoon J, Choi SC, Park CY, Choi JH, Kim YI, Shim WJ, Lim DS.
Department of Cardiology, College of Medicine, Korea University, Seoul 136-705, Korea.
It has been reported that bone marrow (BM)-side population (SP) cells, with hematopoietic stem cell activity, can transdifferentiate into cardiomyocytes and contribute to myocardial repair. However, this has been questioned by recent studies showing that hematopoietic stem cells (HSCs) adopt a hematopoietic cell lineage in the ischemic myocardium. The present study was designed to investigate whether BM-SP cells can in fact transdifferentiate into functional cardiomyocytes. Phenotypically, BM-SP cells were 19.59% (+/-)9.00 CD14+, 8.22% (+/-)2.72 CD34+, 92.93% (+/-)2.68 CD44+, 91.86% (+/-)4.07 CD45+, 28.48% (+/-)2.24 c-kit+, 71.09% (+/-)3.67 Sca-1+. Expression of endothelial cell markers (CD31, Flk-1, Tie-2 and VEGF-A) was higher in BM-SP cells than whole BM cells. After five days of co-culture with neonatal cardiomyocytes, 7.2% (+/-)1.2 of the BM-SP cells expressed sarcomeric -actinin as measured by flow cytometry. Moreover, BM-SP cells co-cultured on neonatal cardiomyocytes fixed to inhibit cell fusion also expressed sarcomeric -actinin. The co-cultured BM-SP cells showed neonatal cardiomyocyte-like action potentials of relatively long duration and shallow resting membrane potential. They also generated calcium transients with amplitude and duration similar to those of neonatal cardiomyocytes. These results show that BM-SP cells are capable of functional cardiomyogenic differentiation when co-cultured with neonatal cardiomyocytes.
＜引用終わり＞
それで、なんで素粒子に関心があるかと言うと、量子力学の観測問題というのがあって、観測すると位置が決まる、観測前はどこにおるか決められん（わからん、でなく、きめられんです）
これは幹細胞に似てるわけ。ヒエラルキー論では決定論的なんですが、実体としての幹細胞は場の関数でもあり、観測の関数でもあり、時間の関数でもあり、決定論にさからっとるわけだ。「何になろうと俺の勝手や、もんく言うな」、と言ってるみたい。
素朴な物心２言論が量子概念ですっ飛んだように、まさに幹細胞概念も秦の始皇帝に始まるヒエラルキードグマに反抗している！？
だから医学も、新時代なのだわ。
投稿者: ojisan
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